Confocal stereology: an efficient tool for measurement of microscopic structures

0444183 - FGÚ 2016 RIV DE eng J - Článek v odborném periodiku
Kubínová, Lucie - Janáček, Jiří
Confocal stereology: an efficient tool for measurement of microscopic structures.
Cell and Tissue Research. Roč. 360, č. 1 (2015), s. 13-28. ISSN 0302-766X. E-ISSN 1432-0878
Grant CEP: GA MŠMT(CZ) LH13028
Institucionální podpora: RVO:67985823
Klíčová slova: 3-D images * confocal microscopy * geometrical characteristics * spatial probes * stereology
Kód oboru RIV: EA - Morfologické obory a cytologie
Impakt faktor: 2.948, rok: 2015

Confocal stereology, a contemporary approach to evaluation of microscopic structures by using a combination of stereological methods and confocal microscopy, uses 3-D images acquired by confocal microscopy for estimation of geometrical characteristics of microscopic structures by stereological methods based on evaluation of optical sections within a thick slice using computer-generated virtual test probes. Volume, number of objects, surface area and length of the structure under study are estimated using interactively evaluated interactions with relevant spatial probes. Overview of the methods of confocal stereology developed during past 30 years is presented and their advantages and pitfalls in comparison with other methods for measurement of geometrical characteristics of microscopic structure are discussed
Trvalý link: http://hdl.handle.net/11104/0246766