Polymerase synthesis of DNA labelled with benzylidene cyanoacetamide-based fluorescent molecular rotors: fluorescent light-up probes for DNA-binding proteins

0443739 - ÚOCHB 2016 RIV GB eng J - Článek v odborném periodiku
Dziuba, Dmytro - Pohl, Radek - Hocek, Michal
Polymerase synthesis of DNA labelled with benzylidene cyanoacetamide-based fluorescent molecular rotors: fluorescent light-up probes for DNA-binding proteins.
Chemical Communications. Roč. 51, č. 23 (2015), s. 4880-4882. ISSN 1359-7345. E-ISSN 1364-548X
Grant CEP: GA ČR GBP206/12/G151
Institucionální podpora: RVO:61388963
Klíčová slova: enzyme amplification reaction * modified oligonucleotides * nucleoside triphosphates
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 6.567, rok: 2015
http://pubs.rsc.org/en/content/articlepdf/2015/cc/c5cc00530b

Viscosity-sensitive fluorophores, fluorescent molecular rotors based on aminobenzylidene-cyanoacetamide moiety, were tethered to 2'-deoxycytidine triphosphate via a propargylamine linker and incorporated into DNA by polymerases in primer extension, nicking enzyme amplification or PCR. DNA probes incorporating modified nucleosides show a light-up response upon binding to a protein.
Trvalý link: http://hdl.handle.net/11104/0246447