SART3-Dependent Accumulation of Incomplete Spliceosomal snRNPs in Cajal Bodies

0442411 - ÚMG 2015 RIV US eng J - Článek v odborném periodiku
Novotný, Ivan - Malinová, Anna - Stejskalová, Eva - Matějů, Daniel - Klimešová, Klára - Roithová, Adriana - Švéda, M. - Knejzlík, Z. - Staněk, David
SART3-Dependent Accumulation of Incomplete Spliceosomal snRNPs in Cajal Bodies.
Cell Reports. Roč. 10, č. 3 (2015), s. 429-440. ISSN 2211-1247. E-ISSN 2211-1247
Grant CEP: GA MŠMT LH14033; GA AV ČR M200521206; GA ČR GAP302/11/1910
Institucionální podpora: RVO:68378050
Klíčová slova: Cajal body * snRNP * surveillance
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 7.870, rok: 2015

Cajal bodies (CBs) are evolutionarily conserved nuclear structures involved in the metabolism of spliceosomal small nuclear ribonucleoprotein particles (snRNPs). CBs are not present in all cell types, and the trigger for their formation is not yet known. Here, we depleted cells of factors required for the final steps of snRNP assembly and assayed for the presence of stalled intermediates in CBs. We show that depletion induces formation of CBs in cells that normally lack these nuclear compartments, suggesting that CB nucleation is triggered by an imbalance in snRNP assembly. Accumulation of stalled intermediates in CBs depends on the di-snRNP assembly factor SART3. SART3 is required for both the induction of CB formation as well as the tethering of incomplete snRNPs to coilin, the CB scaffolding protein. We propose a model wherein SART3 monitors tri-snRNP assembly and sequesters incomplete particles in CBs, thereby allowing cells to maintain a homeostatic balance of mature snRNPs in the nucleoplasm.
Trvalý link: http://hdl.handle.net/11104/0245214