Seeking Novel Nitrilase Enzyme (Nitrile Aminohydrolase, EC 3.5.5.1)

0441610 - MBÚ 2015 RIV CZ eng V - Výzkumná zpráva
Martínková, Ludmila - Kaplan, Ondřej - Veselá, Alicja Barbara
Seeking Novel Nitrilase Enzyme (Nitrile Aminohydrolase, EC 3.5.5.1).
Praha: Innocentive Inc, 2014. 3 s. ISBN N. ISSN N
Institucionální podpora: RVO:61388971
Klíčová slova: Nitrilase * Expression * Purification
Kód oboru RIV: CE - Biochemie

Our research group has created a library of novel nitrilases. In general, the enzymes are available from heterologous producers in amounts ranging from 10 to 20 mg of protein exhbiting 25-50 % purity. The enzymes differ from each other in substrate specificity, temperature optimum, enantioselectivity (arylacetonitrilases) etc. Preferential substrates are phenylacetonitrile, (R,S)-mandelonitrile, benzonitrile or cyanopyridines (depending on the enzyme).
Trvalý link: http://hdl.handle.net/11104/0244597