Dynamic Structures of DNA Heptamers with Different Central Trinucleotide Sequences Studied by Electrochemical and Spectral Methods

0440601 - BFÚ 2015 RIV DE eng J - Článek v odborném periodiku
Pilarová, I. - Kejnovská, Iva - Vorlíčková, Michaela - Trnková, L.
Dynamic Structures of DNA Heptamers with Different Central Trinucleotide Sequences Studied by Electrochemical and Spectral Methods.
Electroanalysis. Roč. 26, č. 10 (2014), s. 2118-2128. ISSN 1040-0397. E-ISSN 1521-4109
Grant CEP: GA ČR(CZ) GAP205/12/0466
Institucionální podpora: RVO:68081707
Klíčová slova: DNA heptamers * Voltammetry * Elimination voltammetric procedure
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 2.138, rok: 2014

A short, single-stranded DNA fragment d(GCGAAGC) and its analogs d(GCNNNGC) carrying different trinucleotide sequences in the center of the molecule (AAA, CCC, GGG or TTT) were investigated by spectral (CD and UV spectra) and electrochemical methods (voltammetry, polyacrylamide gel electrophoresis). Our results show that (i) the central triplet GAA or AAA dramatically stabilizes DNA hairpins, (ii) a stemloop configuration is not supported by CCC or TTT sequences, instead, these heptamers adopt bimolecular duplex forms. Finally (iii), in the case of GGG sequence a very stable supramolecular G-quadruplex is formed.
Trvalý link: http://hdl.handle.net/11104/0243727