WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins

0440165 - BFÚ 2015 US eng J - Článek v odborném periodiku
Kilander, M.B.C. - Dijksterhuis, J. - Ganji, R.S. - Bryja, Vítězslav - Schulte, G.
WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins.
Cellular Signalling. Roč. 23, č. 3 (2011), s. 550-554. ISSN 0898-6568. E-ISSN 1873-3913
Grant CEP: GA ČR(CZ) GD204/09/H058
Institucionální podpora: RVO:68081707
Klíčová slova: COUPLED RECEPTORS * INTERNATIONAL UNION * FRIZZLED RECEPTORS
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 4.058, rok: 2011

The lipoglycoproteins of the WNT family act on seven transmembrane-spanning Class Frizzled receptors. Here, we show that WNT-5A evokes a proliferative response in a mouse microglia-like cell line (N13), which is sensitive to pertussis toxin, thus implicating the involvement of heterotrimeric G proteins of the G(i/o) family. We continue to show that WNT-5A stimulation of N13 membranes and permeabilized cells evokes the exchange of GDP for GTP of pertussis toxin-sensitive G proteins employing [gamma-(35)S]GTP assay and activity state-specific antibodies to GTP-bound G(i) proteins. Our functional analysis of the PTX-sensitivity of WNT-induced G protein activation and PCR analysis of G protein and FZD expression patterns suggest that WNT-5A stimulation leads to the activation of G(i2/3) proteins in N13 cells possibly mediated by FZD(5), the predominant FZD expressed.
Trvalý link: http://hdl.handle.net/11104/0243304