Low molecular weight hyaluronan mediated CD44 dependent induction of IL-6 and chemokines in human dermal fibroblasts potentiates innate immune response

0440160 - BFÚ 2015 RIV GB eng J - Článek v odborném periodiku
Vištejnová, L. - Šafránková, B. - Nešporová, K. - Slavkovský, R. - Hermanová, M. - Hošek, P. - Velebný, V. - Kubala, Lukáš
Low molecular weight hyaluronan mediated CD44 dependent induction of IL-6 and chemokines in human dermal fibroblasts potentiates innate immune response.
Cytokine. Roč. 70, č. 2 (2014), s. 97-103. ISSN 1043-4666. E-ISSN 1096-0023
Grant CEP: GA MŠMT(CZ) ED1.100/02/0123
Institucionální podpora: RVO:68081707
Klíčová slova: COLLAGEN-INDUCED ARTHRITIS * COLLAGEN-INDUCED ARTHRITIS * IN-VITRO
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 2.664, rok: 2014

Complex regulation of the wound healing process involves multiple interactions among stromal tissue cells, inflammatory cells, and the extracellular matrix. Low molecular weight hyaluronan (LMW HA) derived from the degradation of high molecular weight hyaluronan (HMW HA) is suggested to activate cells involved in wound healing through interaction with HA receptors. In particular, receptor CD44 is suggested to mediate cell response to HA of different MW, being the main cell surface HA receptor in stromal tissue and immune cells. However, the response of dermal fibroblasts, the key players in granulation tissue formation within the wound healing process, to LMW HA and their importance for the activation of immune cells is unclear. In this study we show that LMW HA (4.3 kDa) induced pro-inflammatory cytokine IL-6 and chemokines IL-8, CXCL1, CXCL2, CXCL6 and CCL8 gene expression in normal human dermal fibroblasts (NHDF) that was further confirmed by increased levels of IL-6 and IL-8 in cell culture supernatants.
Trvalý link: http://hdl.handle.net/11104/0243303