Defensins from the tick Ixodes scapularis are effective against phytopathogenic fungi and the human bacterial pathogen Listeria grayi

0439571 - BC 2015 RIV GB eng J - Článek v odborném periodiku
Tonk, Miray - Cabezas-Cruz, A. - Valdés, James J. - Rego, Ryan O. M. - Chrudimská, Tereza - Strnad, Martin - Šíma, Radek - Bell-Sakyi, L. - Franta, Z. - Vilcinskas, A. - Grubhoffer, Libor - Rahnamaeian, M.
Defensins from the tick Ixodes scapularis are effective against phytopathogenic fungi and the human bacterial pathogen Listeria grayi.
Parasites & Vectors. Roč. 7, DEC 3 2015 (2014), s. 554. ISSN 1756-3305. E-ISSN 1756-3305
Grant CEP: GA ČR(CZ) GAP302/11/1901; GA MŠMT(CZ) EE2.3.30.0032; GA ČR GP13-12816P
Institucionální podpora: RVO:60077344
Klíčová slova: Antimicrobial peptide * Defensin * Listeria grayi * Fusarium spp * Ixodes scapularis * Tick cell line
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 3.430, rok: 2014

Scapularisin-6 efficiently killed L. grayi, and both Scapularisin-3 and Scapularisin-6 caused strong inhibition (IC50 value: ~1 μM) of the germination of plant-pathogenic fungi Fusarium culmorum and Fusarium graminearum. Scapularisin-6 gene expression was observed in I. scapularis salivary glands and midgut. However, Scapularisin-3 gene expression was only detected in the salivary glands. Transcripts from the two defensins were not found in the I. scapularis tick cell lines ISE6 and ISE18.
Trvalý link: http://hdl.handle.net/11104/0242829