Characterization of a model cyanobacterium Synechocystis sp. PCC 6803 autotrophic growth in a flat-panel photobioreactor

0438456 - ÚVGZ 2016 RIV DE eng J - Článek v odborném periodiku
Zavřel, T. - Sinětova, M. A. - Búzová, Diana - Literáková, Petra - Červený, Jan
Characterization of a model cyanobacterium Synechocystis sp. PCC 6803 autotrophic growth in a flat-panel photobioreactor.
Engineering in Life Sciences. Roč. 15, č. 1 (2015), s. 122-132. ISSN 1618-0240. E-ISSN 1618-2863
Grant CEP: GA MŠMT(CZ) ED1.1.00/02.0073; GA MŠMT(CZ) EE2.3.20.0256
Institucionální podpora: RVO:67179843
Klíčová slova: Carbon dioxide * Exponential phase * Growth optimization * Light * Temperature
Kód oboru RIV: EH - Ekologie - společenstva
Impakt faktor: 2.168, rok: 2015

We characterized the photoautotrophic growth of glucose-tolerant Synechocystis sp. PCC 6803 in a flat-panel photobioreactor running on a semicontinuous regime under various lights, temperatures, and influx carbon dioxide concentrations. The maximum reached growth rate was 0.135 h−1, which corresponds to a doubling time of 5.13 h—a growth speed never reported for Synechocystis before. Saturating red light intensity for the strain was 220–360 μmol(photons) m−2 s−1, and we did not observe any photoinhibition up to 660 μmol(photons) m−2 s−1. Synechocystis was able to grow under red light only; however, photons of wavelengths 405–585 and 670–700 nm further improved its growth. Optimal growth temperature was 35°C. Below 32°C, the growth rates decreased linearly with temperature coefficient (Q10) 1.70. Semicontinuous cultivation is known to be efficient for growth characterization and optimization. However, the assumption of correct growth rates calculation— culture exponential growth—is often not fulfilled. The semicontinuous setup in this study was operated as a turbidostat. Accurate online OD measurements with high time-resolution allowed fast and reliable growth rates determination. Repeating diluting frequencies (up to 18 dilutions per day) were essential for rapid growth stability evaluation. The presented setup provides improvement to previously published semicontinuous characterization strategies by decreasing experimental time requirements and maintaining the culture in exponential growth phase throughout the entire characterization procedure.
Trvalý link: http://hdl.handle.net/11104/0241928