Deposition of thin titanium-copper films with antimicrobial effect by advanced magnetron sputtering methods

0438450 - FZÚ 2015 RIV NL eng J - Článek v odborném periodiku
Straňák, V. - Wulff, H. - Rebl, H. - Zietz, C. - Arndt, K. - Bogdanowicz, R. - Nebe, B. - Bader, R. - Podbielski, A. - Hubička, Zdeněk - Hippler, R.
Deposition of thin titanium-copper films with antimicrobial effect by advanced magnetron sputtering methods.
Materials Science & Engineering C-Materials for Biological Applications. Roč. 31, č. 7 (2011), s. 1512-1519. ISSN 0928-4931. E-ISSN 1873-0191
Grant CEP: GA ČR(CZ) GAP205/11/0386; GA MŠMT(CZ) 1M06002
Výzkumný záměr: CEZ:AV0Z10100520
Klíčová slova: implant coating * titanium-copper film * pulsed magnetron sputtering
Kód oboru RIV: BM - Fyzika pevných látek a magnetismus
Impakt faktor: 2.686, rok: 2011

The antibacterial effect of thin titanium-copper (Ti-Cu) films combined with sufficient growth of human osteoblastic cells is reported in the paper. Thin Ti-Cu films were prepared by three different plasma-assisted magnetron sputtering methods: direct current magnetron sputtering (dc-MS), dual magnetron sputtering (dual-MS) as well as dual high power impulse magnetron sputtering (dual-HiPIMS). The antimicrobial effect is caused by copper released from the metallic Ti-Cu films, which was measured by atomic absorption spectroscopy (AAS). The copper release is influenced by the chemical and physical properties of the deposited films and was investigated by X-ray diffractometry and X-ray reflectometry (GIXD and XR) techniques. It was found that, within the first 24 h the amount of Cu released from dual-HiPIMS films (about 250 mu g) was much higher than from dc-MS and dual-MS films. In vitro planktonic growth tests on Ti-Cu surfaces for Staphylococcus epidermidis and S. aureus demonstrated the killing of both bacteria using the Ti-Cu films prepared using the dual-HiPIMS technique. The killing effects on biofilm bacteria were less obvious. After the total release of copper from the Ti-Cu film the vitality of exposed human osteoblast MG-63 cells increased significantly. An initial cytotoxic effect followed by the growth of osteoblastic cells was demonstrated. The cytotoxic effect combined with growth of osteoblastic cells could be used in joint replacement surgery to reduce the possibility of infection and to increase adoption of the implants.
Trvalý link: http://hdl.handle.net/11104/0241858