Methoxyphenol and Dihydrobenzofuran as Oxidizable Labels for Electrochemical Detection of DNA

0438294 - ÚOCHB 2015 RIV DE eng J - Článek v odborném periodiku
Simonova, Anna - Balintová, Jana - Pohl, Radek - Havran, Luděk - Fojta, Miroslav - Hocek, Michal
Methoxyphenol and Dihydrobenzofuran as Oxidizable Labels for Electrochemical Detection of DNA.
ChemPlusChem. Roč. 79, č. 12 (2014), s. 1703-1712. ISSN 2192-6506. E-ISSN 2192-6506
Grant CEP: GA ČR GBP206/12/G151
Institucionální podpora: RVO:61388963 ; RVO:68081707
Klíčová slova: DNA * electrochemistry * nucleotides * polymerase chain reactions * redox labeling
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 3.026, rok: 2014

4-Hydroxy-3-methoxyphenyl (MOP) and 2,3-dihydrobenzofuran-5-yl (DHB) groups were tested as potential new oxidizable labels for electrochemical detection of DNA. The corresponding 5-aryl-cytosine and 7-aryl-7-deazaadenine 2-deoxyribonucleoside triphosphates (dNTPs) were prepared by the Suzuki coupling of halogenated dNTPs or by triphosphorylation of modified nucleosides, and were incorporated enzymatically into DNA by polymerase. In parallel, the electrochemical behavior of the labeled nucleosides, nucleotides, and DNA was studied to show that most of them give analytically useful signals of oxidation on the carbon electrode. Particularly, MOP appears to be a useful oxidizable DNA label since it offers independent detection of the modified DNA.
Trvalý link: http://hdl.handle.net/11104/0241841