Potato virus X induces DNA damage in leaf nuclei of the host plant Nicotiana tabacum L. var. xanthi

0438179 - ÚEB 2015 RIV NL eng J - Článek v odborném periodiku
Čeřovská, Noemi - Plchová, Helena - Vaculík, Petr - Moravec, Tomáš - Gichner, Tomáš
Potato virus X induces DNA damage in leaf nuclei of the host plant Nicotiana tabacum L. var. xanthi.
Biologia Plantarum. Roč. 58, č. 4 (2014), s. 783-787. ISSN 0006-3134. E-ISSN 1573-8264
Grant CEP: GA ČR(CZ) GAP501/12/1761
Institucionální podpora: RVO:61389030
Klíčová slova: Comet assay * ethyl methanesulphonate * single cell gel electrophoresis
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 1.849, rok: 2014

We employed the comet assay (single cell gel electrophoresis) to evaluate induced DNA damage in nuclei isolated from tobacco leaves (Nicotiana tabacum var. xanthi) inoculated with Potato virus X (PVX). The highest DNA damage, expressed by the tail moment value, was observed in the inoculated leaves and decreased in the 1(st) to 4(th) systemic leaves. DNA damage increased with the time after the inoculation (from day 3 to day 21) and was higher in nuclei isolated from a part of the leaf at the petiole compared to nuclei isolated from the leaf tip. A Pearson moment correlation (r = 0.94) between the induced DNA damage and the PVX titres expressed by ELISA absorbance values was observed. The PVX infection did not induce a significant increase in the rate of somatic mutations evaluated by appearance of dark green, yellow, and double green/yellow sectors on the heterozygous pale green leaves of N. tabacum var. xanthi.
Trvalý link: http://hdl.handle.net/11104/0241644