Počet záznamů: 1  

Murine cytomegalovirus is not restricted to the house mouse Mus musculus domesticus: prevalence and genetic diversity in the European house mouse hybrid zone

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    0437899 - ÚBO 2015 RIV US eng J - Článek v odborném periodiku
    Goüy de Bellocq, Joëlle - Baird, Stuart J. E. - Albrechtová, Jana - Sobeková, Karolina - Piálek, Jaroslav
    Murine cytomegalovirus is not restricted to the house mouse Mus musculus domesticus: prevalence and genetic diversity in the European house mouse hybrid zone.
    Journal of Virology. Roč. 89, č. 1 (2015), s. 406-414. ISSN 0022-538X. E-ISSN 1098-5514
    Grant CEP: GA ČR GA206/08/0640; GA ČR(CZ) GA14-35009S
    Institucionální podpora: RVO:68081766
    Klíčová slova: maximum likelihood * viral antibodies * wild mice * strains * recombination * mitochondrial * Australia * evolution * virus * model
    Kód oboru RIV: EG - Zoologie
    Impakt faktor: 4.606, rok: 2015

    Murine cytomegalovirus (MCMV) is a betaherpesvirus of the house mouse, Mus musculus domesticus. It is a common infectious agent of wild mice and a highly studied pathogen of the laboratory mouse. Betaherpesviruses are specific to their hosts, and it is not known if other Mus taxa carry MCMV or if it is restricted to M. m. domesticus. We sampled mice over a 145-km transect of Bavaria-Bohemia crossing a hybrid zone between M. m. domesticus and Mus musculus musculus in order to investigate the occurrence of MCMV in two Mus subspecies and to test the limits of the specificity of the virus for its host. We hypothesized that if the two subspecies carry MCMV and if the virus is highly specific to its host, divergent MCMV lineages would have codiverged with their hosts and would have a geographical distribution constrained by the host genetic background. A total of 520 mice were tested by enzyme-linked immunosorbent assay (ELISA) and/or nested PCR targeting the M94 gene. Seropositive and PCR-positive individuals were found in both Mus subspecies. Seroprevalence was high, at 79.4%, but viral DNA was detected in only 41.7% of mice. Sequencing revealed 20 haplotypes clustering in 3 clades that match the host genetic structure in the hybrid zone, showing 1 and 2 MCMV lineages in M. m. domesticus and M. m. musculus, respectively. The estimated time to the most recent common ancestor (1.1 million years ago [Mya]) of the MCMVs matches that of their hosts. In conclusion, MCMV has coevolved with these hosts, suggesting that its diversity in nature may be underappreciated, since other members of the subgenus Mus likely carry different MCMVs.
    Trvalý link: http://hdl.handle.net/11104/0241377

     
     
Počet záznamů: 1  

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