ROS production in brown adipose tissue mitochondria: The question of UCP1-dependence

0437819 - FGÚ 2015 RIV NL eng J - Článek v odborném periodiku
Shabalina, I.G. - Vrbacký, Marek - Pecinová, Alena - Kalinovich, A. V. - Drahota, Zdeněk - Houštěk, Josef - Mráček, Tomáš - Cannon, B. - Nedergaard, J.
ROS production in brown adipose tissue mitochondria: The question of UCP1-dependence.
Biochimica Et Biophysica Acta-Bioenergetics. Roč. 1837, č. 12 (2014), s. 2017-2030. ISSN 0005-2728. E-ISSN 1879-2650
Grant CEP: GA MŠMT(CZ) LL1204; GA ČR(CZ) GB14-36804G
Institucionální podpora: RVO:67985823
Klíčová slova: reactive oxygen species * uncoupling protein 1 * brown adipose tissue mitochondria * cold acclimation * glycerol-3-phosphate dehydrogenase (EC 1.1.5.3) * succinate
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 5.353, rok: 2014

Whether active UCP1 can reduce ROS production in brown-fat mitochondria is presently not settled. The significance of UCP1 for ROS production was studied by comparing the ROS production in brown-fat mitochondria isolated from wildtype mice or from UCP1(−/−) knockout mice. Only ROS production supported by exogenously added succinate was affected by the presence of active UCP1; ROS production supported by any other tested substrate was unaffected. In conclusion, UCP1 activity does not generally diminish ROS production in brown-fat mitochondria
Trvalý link: http://hdl.handle.net/11104/0241351