Raman tweezers in microfluidic systems for analysis and sorting of living cells

0437742 - ÚPT 2015 RIV US eng C - Konferenční příspěvek (zahraniční konf.)
Pilát, Zdeněk - Ježek, Jan - Kaňka, Jan - Zemánek, Pavel
Raman tweezers in microfluidic systems for analysis and sorting of living cells.
19th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics (Proceedings of SPIE 9441). Bellingham: SPIE, 2014, 944107:1-8. ISBN 9781628415568. ISSN 0277-786X.
[Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics /19./. Jelenia Góra (PL), 08.09.2014-12.09.2014]
Grant CEP: GA MPO FR-TI1/433; GA TA ČR TA03010642; GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institucionální podpora: RVO:68081731
Klíčová slova: cells * chemicals * fluorescent markers * optical analysis * optical trappingo * organisms * Raman spectroscopy * proteins
Kód oboru RIV: BH - Optika, masery a lasery

We have devised an analytical and sorting system combining optical trapping with Raman spectroscopy in microfluidic environment, dedicated to identification and sorting of biological objects, such as living cells of various unicellular organisms. Our main goal was to create a robust and universal platform for non-destructive and non-contact sorting of micro-objects based on their Raman spectral properties. This approach allowed us to collect spectra containing information about the chemical composition of the objects, such as the presence and composition of pigments, lipids, proteins, or nucleic acids, avoiding artificial chemical probes such as fluorescent markers. The non-destructive nature of this optical analysis and manipulation allowed us to separate individual living cells of our interest in a sterile environment and provided the possibility to cultivate the selected cells for further experiments. We used a mixture of polystyrene micro-particles and algal cells to test and demonstrate the function of our analytical and sorting system. The devised system could find its use in many medical, biotechnological, and biological applications.
Trvalý link: http://hdl.handle.net/11104/0241262