Sensitive detection of interferon-gamma with engineered proteins and surface plasmon resonance biosensor

0437424 - ÚFE 2015 RIV NL eng C - Konferenční příspěvek (zahraniční konf.)
Šípová, Hana - Ševců, Veronika - Kuchař, Milan - Ahmad, Jawid Nazir - Mikulecký, Pavel - Šebo, Peter - Malý, Petr - Homola, Jiří
Sensitive detection of interferon-gamma with engineered proteins and surface plasmon resonance biosensor.
Procedia Engineering. Vol. 25. Amsterdam: Elsevier, 2011 - (Tsamis, C.; Kaltsas, G.), s. 940-943. ISBN 978-1-62748-581-4. ISSN 1877-7058.
[25th Eurosensors Conference. Athens (GR), 04.09.2011-07.09.2011]
Grant CEP: GA AV ČR KAN200670701; GA AV ČR KAN200520702; GA MŠMT OC09058
Výzkumný záměr: CEZ:AV0Z20670512; CEZ:AV0Z50520701; CEZ:AV0Z50200510
Klíčová slova: Surface plasmon resonance * Biosensor * Interferon gamma
Kód oboru RIV: JA - Elektronika a optoelektronika, elektrotechnika; EI - Biotechnologie a bionika (BTO-N); CE - Biochemie (MBU-M)

We present a novel surface plasmon resonance (SPR) biosensor for detection of human interferon gamma (hIFNy), an important diagnostic marker for several infectious diseases. The biosensor employs engineered proteins derived from albumin binding domain (ABD) and selected with ribosome display to provide high affinity towards hIFNy. We demonstrate that the biosensor provides rapid, sensitive, and cost-effective platform for the detection of hIFNγ and allows its detection at nanomolar levels both in buffer and diluted human plasma
Trvalý link: http://hdl.handle.net/11104/0241007