Biosensors with nuclear tracks and embedded membranes

0437195 - ÚJF 2015 CH eng C - Konferenční příspěvek (zahraniční konf.)
Fink, Dietmar - Vacík, Jiří - García Arellano, H. - Munoz, G. H. - Alfonta, L. - Fahrner, W. R. - Hoppe, K. - Kiv, A.
Biosensors with nuclear tracks and embedded membranes.
Key Engineering Materials. Vol. 605. Cürich: Transt Tech Publications Ltd, 2014, s. 83-86. ISBN 978-303835051-4. ISSN 1013-9826.
[3rd International Conference on Materials and Applications for Sensors and Transducers. Praha (CZ), 13.09.2013-17.09.2013]
Grant CEP: GA MŠMT LM2011019
Institucionální podpora: RVO:61389005
Klíčová slova: Biosensors * Coupled chemical reactions * Enzymes * Etching * Ion irradiation * Membranes * Nuclear tracks * Polymers * Precipitation
Kód oboru RIV: BM - Fyzika pevných látek a magnetismus

One of the strategies for enhancing the detection sensitivity of etched nuclear track-based biosensors uses the cladding of track walls with enzymes and the subsequent enrichment of charged enzymatic reaction products in the confined volume of the narrow tracks. For sensing two different bio-molecule types simultaneously, the tracks can be separated into two adjacent compartments by inserting a membrane in between them. The recipe for membrane formation is outlined. If the same enzyme is inserted into both compartments, the sensing accuracy can greatly be enhanced, as compared to etched tracks without membranes, due to a different underlying sensing mechanism. Such sensors act as frequency filters, which is useful for sensors operating in electrosmog-rich ambient.
Trvalý link: http://hdl.handle.net/11104/0240827