Epigenetic regulations in the IFNγ signalling pathway: IFNγ-mediated MHC class I upregulation on tumour cells is associated with DNA demethylation of antigen-presenting machinery genes

0436793 - ÚMG 2015 RIV US eng J - Článek v odborném periodiku
Vlková, Veronika - Štěpánek, Ivan - Hrušková, Veronika - Šenigl, Filip - Mayerová, Veronika - Šrámek, Martin - Šímová, Jana - Bieblová, Jana - Indrová, Marie - Hejhal, Tomáš - Dérian, N. - Klatzmann, D. - Six, A. - Reiniš, Milan
Epigenetic regulations in the IFNγ signalling pathway: IFNγ-mediated MHC class I upregulation on tumour cells is associated with DNA demethylation of antigen-presenting machinery genes.
OncoTarget. Roč. 5, č. 16 (2014), s. 6923-35. ISSN 1949-2553
Grant CEP: GA ČR GAP301/10/2174; GA MZd NT14461
GRANT EU: European Commission(XE) 18933 - CLINIGENE
Grant ostatní: French state funds within the Investissements d’Avenir program(FR) ANR-11-IDEX-0004-02
Institucionální podpora: RVO:68378050
Klíčová slova: IFNγ signalling pathway * DNA demethylation * tumour
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 6.359, rok: 2014

Downregulation of MHC class I expression on tumour cells, a common mechanism by which tumour cells can escape from specific immune responses, can be associated with coordinated silencing of antigen-presenting machinery genes. The expression of these genes can be restored by IFNγ. In this study we documented association of DNA demethylation of selected antigen-presenting machinery genes located in the MHC genomic locus (TAP-1, TAP-2, LMP-2, LMP-7) upon IFNγ treatment with MHC class I upregulation on tumour cells in several MHC class I-deficient murine tumour cell lines (TC-1/A9, TRAMP-C2, MK16 and MC15). Our data also documented higher methylation levels in these genes in TC-1/A9 cells, as compared to their parental MHC class I-positive TC-1 cells. IFNγ-mediated DNA demethylation was relatively fast in comparison with demethylation induced by DNA methyltransferase inhibitor 5-azacytidine, and associated with increased histone H3 acetylation in the promoter regions of APM genes. Comparative transcriptome analysis in distinct MHC class I-deficient cell lines upon their treatment with either IFNγ or epigenetic agents revealed that a set of genes, significantly enriched for the antigen presentation pathway, was regulated in the same manner. Our data demonstrate that IFNγ acts as an epigenetic modifier when upregulating the expression of antigen-presenting machinery genes.
Trvalý link: http://hdl.handle.net/11104/0241959