Sensitivity of bacteria to diamond nanoparticles of various size differs in gram-positive and gram-negative cells

0436429 - FZÚ 2015 RIV NL eng J - Článek v odborném periodiku
Beranová, Jana - Seydlová, Gabriela - Kozak, Halyna - Benada, Oldřich - Fišer, R. - Artemenko, Anna - Konopásek, I. - Kromka, Alexander
Sensitivity of bacteria to diamond nanoparticles of various size differs in gram-positive and gram-negative cells.
FEMS Microbiology Letters. Roč. 351, č. 2 (2014), s. 179-186. ISSN 0378-1097. E-ISSN 1574-6968
Grant CEP: GA ČR GAP108/12/0910; GA ČR GPP205/12/P331
Institucionální podpora: RVO:68378271 ; RVO:61388971
Klíčová slova: diamond nanoparticles * antibacterial properties * Escherichia coli * Bacillus subtilis * DLS * XPS
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 2.121, rok: 2014

The influence of the size and surface termination of diamond nanoparticles (DNPs) on their antibacterial activity against Escherichia coli and Bacillus subtilis was assessed. The average size and distribution of DNPs were determined by dynamic light scattering and X-ray diffraction techniques. The chemical composition of the DNPs studied by X-ray photoelectron spectroscopy showed that DNPs >5 nm and oxidized particles have a higher oxygen content. The antibacterial potential of DNPs was assessed by the viable count method. In general, E. coli exhibited a higher sensitivity to DNPs than B. subtilis. However, in the presence of all the DNPs tested, the B. subtilis colonies exhibited altered size and morphology. Antibacterial activity was influenced not only by DNP concentration but also by DNP size and form.
Trvalý link: http://hdl.handle.net/11104/0240176