The comparative profile of lymphoid cells and the T and B cell spectratype of germ-free piglets infected with viruses SIV, PRRSV or PCV2

0435549 - MBÚ 2015 RIV FR eng J - Článek v odborném periodiku
Šinkora, Marek - Butler, J. E. - Lager, K. - Potočková, Hana - Šinkorová, Jana
The comparative profile of lymphoid cells and the T and B cell spectratype of germ-free piglets infected with viruses SIV, PRRSV or PCV2.
Veterinary Research. Roč. 45, SEP 2014 (2014). ISSN 0928-4249. E-ISSN 1297-9716
Grant CEP: GA ČR GAP502/12/0110
Institucionální podpora: RVO:61388971
Klíčová slova: RESPIRATORY SYNDROME VIRUS * PORCINE CIRCOVIRUS TYPE-2 * ANTIBODY REPERTOIRE DEVELOPMENT
Kód oboru RIV: EC - Imunologie
Impakt faktor: 2.815, rok: 2014

Lymphocyte subsets isolated from germ-free piglets experimentally infected with swine influenza virus (SIV), porcine reproductive and respiratory syndrome virus (PRRSV) or porcine circovirus type 2 (PCV2) were studied and the profile of these subsets among these three infections was monitored. Germ-free piglets were used since their response could be directly correlated to the viral infection. Because SIV infections are resolved even by colostrum-deprived neonates whereas PRRSV and PCV2 infections are not, SIV was used as a benchmark for an effectively resolved viral infection. PRRSV caused a large increase in the proportion of lymphocytes at the site of infection and rapid differentiation of B cells leading to a high level of Ig-producing cells but a severe reduction in CD2(-)CD21(+) primed B cells. Unlike SIV and PCV2, PRRSV also caused an increase in terminally differentiated subset of CD2(+)CD8 alpha(+) gamma delta cells and polyclonal expansion of major V beta families suggesting that non-specific helper T cells drive swift B cell activation. Distinct from infections with SIV and PRRSV, PCV2 infection led to the: (a) prevalence of MHC-II+ T cytotoxic cells, (b) restriction of the T helper compartment in the respiratory tract, (c) generation of a high proportion of FoxP3(+) T cells in the blood and (d) selective expansion of IgA and IgE suggesting this virus elicits a mucosal immune response. Our findings suggest that PRRSV and PCV2 may negatively modulate the host immune system by different mechanisms which may explain their persistence.
Trvalý link: http://hdl.handle.net/11104/0239463