Microarray analysis of serum mRNA in patients with head and neck squamous cell carcinoma at whole-genome scale

0435343 - ÚMG 2015 RIV US eng J - Článek v odborném periodiku
Čapková, M. - Šáchová, Jana - Strnad, Hynek - Kolář, Michal - Hroudová, Miluše - Chovanec, M. - Čada, Z. - Štefl, M. - Valach, J. - Kastner, J. - Smetana, K. Jr. - Plzák, J.
Microarray analysis of serum mRNA in patients with head and neck squamous cell carcinoma at whole-genome scale.
BioMed Research International. -, April 23 (2014). ISSN 2314-6133. E-ISSN 2314-6141
Grant CEP: GA MZd(CZ) NT13488
Institucionální podpora: RVO:68378050
Klíčová slova: Microarray Analysis * Head and Neck Squamous Cell Carcinoma * whole-genome scale
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 1.579, rok: 2014

With the increasing demand for noninvasive approaches in monitoring head and neck cancer, circulating nucleic acids have been shown to be a promising tool. We focused on the global transcriptome of serum samples of head and neck squamous cell carcinoma (HNSCC) patients in comparison with healthy individuals. We compared gene expression patterns of 36 samples. Twenty-four participants including 16 HNSCC patients (from 12 patients we obtained blood samples 1 year posttreatment) and 8 control subjects were recruited. The Illumina HumanWG-6 v3 Expression BeadChip was used to profile and identify the differences in serum mRNA transcriptomes. We found 159 genes to be significantly changed (Storey's P value <0.05) between normal and cancer serum specimens regardless of factors including p53 and B-cell lymphoma family members (Bcl-2, Bcl-XL). In contrast, there was no difference in gene expression between samples obtained before and after surgery in cancer patients. We suggest that microarray analysis of serum cRNA in patients with HNSCC should be suitable for refinement of early stage diagnosis of disease that can be important for development of new personalized strategies in diagnosis and treatment of tumours but is not suitable for monitoring further development of disease.
Trvalý link: http://hdl.handle.net/11104/0241978