Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis

0435141 - MBÚ 2015 RIV GB eng J - Článek v odborném periodiku
Wiedermannová, Jana - Sudzinová, Petra - Koval, Tomáš - Rabatinová, Alžběta - Šanderová, Hana - Ramaniuk, Olga - Rittich, Šimon - Dohnálek, Jan - Fu, Z. - Halada, Petr - Lewis, P. - Krásný, Libor
Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis.
Nucleic Acids Research. Roč. 42, č. 8 (2014), s. 5151-5163. ISSN 0305-1048. E-ISSN 1362-4962
Grant CEP: GA ČR GAP302/11/0855; GA ČR(CZ) GBP305/12/G034; GA ČR GA13-16842S; GA MŠMT(CZ) EE2.3.30.0029; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:61388971 ; RVO:61389013
Klíčová slova: ESCHERICHIA-COLI * BACTERIAL HOMOLOG * DELTA-SUBUNIT
Kód oboru RIV: CE - Biochemie
Impakt faktor: 9.112, rok: 2014

Bacterial RNA polymerase (RNAP) is an essential multisubunit protein complex required for gene expression. Here, we characterize YvgS (HelD) from Bacillus subtilis, a novel binding partner of RNAP. We show that HelD interacts with RNAP-core between the secondary channel of RNAP and the alpha subunits. Importantly, we demonstrate that HelD stimulates transcription in an ATP-dependent manner by enhancing transcriptional cycling and elongation. We demonstrate that the stimulatory effect of HelD can be amplified by a small subunit of RNAP, delta. In vivo, HelD is not essential but it is required for timely adaptations of the cell to changing environment. In summary, this study establishes HelD as a valid component of the bacterial transcription machinery.
Trvalý link: http://hdl.handle.net/11104/0239038