Defensin from the ornate sheep tick Dermacentor marginatus and its effect on Lyme borreliosis spirochetes

0435108 - BC 2015 RIV GB eng J - Článek v odborném periodiku
Chrudimská, Tereza - Čeřovský, Václav - Slaninová, Jiřina - Rego, Ryan O. M. - Grubhoffer, Libor
Defensin from the ornate sheep tick Dermacentor marginatus and its effect on Lyme borreliosis spirochetes.
Developmental and Comparative Immunology. Roč. 46, č. 2 (2014), s. 165-170. ISSN 0145-305X. E-ISSN 1879-0089
Grant CEP: GA ČR(CZ) GAP302/11/1901
Institucionální podpora: RVO:60077344 ; RVO:61388963
Klíčová slova: Tick * Dermacentor marginatus * Defensin * Borrelia afzelii * Antimicrobial activity * Peptide synthesis
Kód oboru RIV: EE - Mikrobiologie, virologie; EE - Mikrobiologie, virologie (UOCHB-X)
Impakt faktor: 2.815, rok: 2014

Expression of the previously reported defensin of the tick Dermacentor marginatus (defDM) was analysed in different organs by RT-PCR. mRNA of the defDM gene was detected in the hemolymph, midgut and salivary glands. Moreover defDM was isolated from the tick hemolymph using RP-HPLC and its sequence was determined by mass spectrometry and Edman degradation. Synthetic peptide was used for determining biological activities. The results showed an anti-Gram-positive bacterial role for the defensin. As D. marginatus ticks appear not to be vectors of the Lyme disease agent of the complex Borrelia burgdorferi sensu lato, we tested the influence of defDM on Borrelia afzelii. There is a very clear borrelicidal activity of the defensin, which is concentration dependent and suggests a possible role in the clearing of Borrelia ingested by D. marginatus ticks.
Trvalý link: http://hdl.handle.net/11104/0239024