Polymerase Synthesis of DNAs Bearing Vinyl Groups in the Major Groove and their Cleavage by Restriction Endonucleases

0435030 - ÚOCHB 2015 RIV DE eng J - Článek v odborném periodiku
Mačková, Michaela - Pohl, Radek - Hocek, Michal
Polymerase Synthesis of DNAs Bearing Vinyl Groups in the Major Groove and their Cleavage by Restriction Endonucleases.
Chembiochem. Roč. 15, č. 15 (2014), s. 2306-2312. ISSN 1439-4227. E-ISSN 1439-7633
Grant CEP: GA ČR GA14-04289S
Institucionální podpora: RVO:61388963
Klíčová slova: DNA polymerases * DNA * DNA-protein interactions * nucleotides * restriction endonucleases
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 3.088, rok: 2014

DNA molecules containing 5-vinyluracil, 5-vinylcytosine, or 7-deaza-7-vinyladenine were prepared by polymerase incorporation of the corresponding vinyl-modified 2-deoxyribonucleoside triphosphates, and the influence of the vinyl group in the major groove of DNA on the cleavage by diverse type II restriction endonucleases (REs) was studied. The presence of 5-vinyluracil was tolerated by most of the REs, whereas only some REs were able to cleave sequences containing 7-deaza-7-vinyladenine. The enzyme ScaI was found to cleave DNA containing 5-vinylcytosine efficiently but not DNA containing the related 5-ethynylcytosine. All other REs failed to cleave sequences containing any cytosine modifications.
Trvalý link: http://hdl.handle.net/11104/0239037