Raman tweezers in microfluidic systems for analysis and sorting of living cells

0434961 - ÚPT 2015 RIV US eng C - Konferenční příspěvek (zahraniční konf.)
Pilát, Zdeněk - Ježek, Jan - Kaňka, Jan - Zemánek, Pavel
Raman tweezers in microfluidic systems for analysis and sorting of living cells.
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII (Proceedings of SPIE 8947). Bellingham: SPIE, 2014, 89471M:1-9. ISBN 9780819498601. ISSN 1605-7422.
[Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues /12./. San Francisco (US), 03.02.2014-06.02.2014]
Grant CEP: GA MPO FR-TI1/433; GA TA ČR TA03010642; GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institucionální podpora: RVO:68081731
Klíčová slova: Chemicals * Fluorescent markers * Optical analysis * Optical trapping * Organisms * Raman spectroscopy
Kód oboru RIV: BH - Optika, masery a lasery

We have devised an analytical and sorting system combining optical trapping with Raman spectroscopy in microfluidic environment in order to identify and sort biological objects, such as living cells of various prokaryotic and eukaryotic organisms. Our main objective was to create a robust and universal platform for non-contact sorting of microobjects based on their Raman spectral properties. This approach allowed us to collect information about the chemical composition of the objects, such as the presence and composition of lipids, proteins, or nucleic acids without using artificial chemical probes such as fluorescent markers. The non-destructive and non-contact nature of this optical analysis and manipulation allowed us to separate individual living cells of our interest in a sterile environment and provided the possibility to cultivate the selected cells for further experiments. We used differently treated cells of algae to test and demonstrate the function of our analytical and sorting system. The devised system could find its use in many medical, biotechnological, and biological applications.
Trvalý link: http://hdl.handle.net/11104/0238902