Determination of free cisplatin in medium by differential pulse polarography after ultrasound and cisplatin treatment of a cancer cell culture

0434830 - BFÚ 2015 eng J - Článek v odborném periodiku
Bernard, V. - Fojt, Lukáš - Škorpíková, J. - Mornstein, V.
Determination of free cisplatin in medium by differential pulse polarography after ultrasound and cisplatin treatment of a cancer cell culture.
Indian Journal of Biochemistry & Biophysics. Roč. 48, č. 1 (2011), s. 59-62. ISSN 0301-1208. E-ISSN 0975-0959
Grant CEP: GA ČR(CZ) GAP205/10/2378
Klíčová slova: Cisplatin * Differential pulse polarography * Ovarian carcinoma cells A2780
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 1.142, rok: 2011

The in vitro study was carried out for detection of the cisplatin in free form and in culture medium, depending on various conditions of sonodynamic human ovarian cancer cells A2780 treatment by differential pulse polarography (DPP). For sonodynamic treatment, we used cisplatin alone and combined cisplatin/ultrasound treatments. The ultrasound exposure intensity of 1.0 and 2.0 W.cm(-2) in far field for incubation periods 1, 24 and 48 h was used.. The parameters of DPP measurements were - 1 s drop time, 5 mV.s(-)1 voltage scan rate, 50 mV modulation amplitude and negative scanning direction; platinum wire served as counter electrode and AglAgCII3 M KCl as reference electrode. The results showed the dependence of free platinum quantities in culture medium on incubation time and treatment protocol. We found difference in concentration of free cisplatin between conventional application of cisplatin and sonodynamic treatment.
Trvalý link: http://hdl.handle.net/11104/0238854