Progress in imaging techniques for characterization of polyelectrolyte complex microcapsules as encapsulation matrice for biocatalysts

0434639 - ÚPT 2015 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Bučko, M. - Gemeiner, P. - Vikartovská, A. - Schenkmayerová, A. - Bertóková, A. - Lacík, I. - Chorvát Jr., D. - Neděla, Vilém
Progress in imaging techniques for characterization of polyelectrolyte complex microcapsules as encapsulation matrice for biocatalysts.
Workshop of Interesting Topics of SEM and ESEM. Brno: Institute of Scientific Instruments AS CR, v. v. i, 2014, s. 36-37. ISBN 978-80-87441-12-1.
[Workshop of Interesting Topics of SEM and ESEM. Mikulov (CZ), 26.08.2014-31.08.2014]
Grant CEP: GA MŠMT EE.2.3.20.0103
Institucionální podpora: RVO:68081731
Klíčová slova: ESEM * matrice for biocatalysts
Kód oboru RIV: JA - Elektronika a optoelektronika, elektrotechnika

Polyelectrolyte complex microcapsules showed to be an univerzal encapsulation matrice for wide range of biocatalysts including viable, native and recombinant bacterial cells, non-viable bacterial cells as well as enzymes. Developed continuous encapsulation protocol based on the polyelectrolyte complexation of oppositely charged polymers enabled production of uniform microcapsules with a controlled shape, size, membrane thickness, permeability and mechanical resistance. The characterisation and control of the microcapsule properties including the inner structure and surface properties was inevitable to determine their proper function and predictability in important applications such as the stabilisation and reuse of encapsulated biocatalysts for more efficient production of valuable compounds. Recent utilisation of unique environmental scanning electron microscope enabled an innovative observation and characterization of capsule morphology in native and fully hydrated state. Achieved results and comparison with previously used conventional microscopic techniques will be presented.
Trvalý link: http://hdl.handle.net/11104/0238650