Prince Cangrande’s Collagen: Study of Protein Modification on the Mummy of the Lord of Verona, Italy (1291–1329 AD)

0434566 - FGÚ 2015 RIV DE eng J - Článek v odborném periodiku
Mikšík, Ivan - Sedláková, Pavla - Pataridis, Statis - Bortolotti, F. - Gottardo, R. - Tagliaro, F.
Prince Cangrande’s Collagen: Study of Protein Modification on the Mummy of the Lord of Verona, Italy (1291–1329 AD).
Chromatographia. Roč. 77, 21-22 (2014), s. 1503-1510. ISSN 0009-5893. E-ISSN 1612-1112
Grant CEP: GA ČR(CZ) GAP206/12/0453; GA ČR(CZ) GA13-17224S
Institucionální podpora: RVO:67985823
Klíčová slova: HPLC/MS * collagen * deamidation * mummy * protein modification
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 1.411, rok: 2014

The natural mummy of prince Cangrande, Lord of Verona, Italy (1291–1329 AD) was studied. The samples were cleaved with trypsin and analysed by liquid chromatographic methods coupled to mass spectrometry (Q-TOF, ion-trap). Special attention was devoted to nonenzymatic protein modification––the deamidation of asparagine and glutamine. A huge amount of collagen was determined in the tissues of the mummy (covering over 80 % of the sequence)––collagen type I was identified in the rib bone and collagen types I and III in the muscle. A high overall percentage of asparaginyl and glutaminyl residues were deamidated (up to 92 %)
Trvalý link: http://hdl.handle.net/11104/0238600