Termination pattern of nanocrystalline diamond controls cell behavior

0434473 - FZÚ 2015 RIV CZ eng A - Abstrakt
Brož, A. - Ukraintsev, Egor - Babchenko, Oleg - Kromka, Alexander - Rezek, Bohuslav - Verdánová, M. - Ostrovská, L. - Sauerová, P. - Hubálek Kalbáčová, M.
Termination pattern of nanocrystalline diamond controls cell behavior.
18th International Microscopy Congress Proceedings. Praha: Czechoslovak microscopy society, 2014 - (Hozák, P.). ISBN 978-80-260-6720-7.
[International Microscopy Congress /18./. 07.09.2014-12.09.2014, Praha]
Grant CEP: GA ČR GAP108/12/0996; GA ČR(CZ) GA14-04790S
Institucionální podpora: RVO:68378271
Klíčová slova: diamond films * patterned surface * surface termination * cell adhesion
Kód oboru RIV: BM - Fyzika pevných látek a magnetismus

The aim of the study was to investigate the behavior of human osteoblastic cells (SAOS-2) on nanocrystalline diamond (NCD) film with altered wettability with a focus on primary adhesion. Surface termination and thus wettability of the NCD was achieved by exposition to oxygen or hydrogen MW plasma. Two different surface termination patterns of the NCD were prepared: 1) half of the surface terminated by O and the other half by H, 2) surface with 200 um wide O/H alternating stripes. Differences in cell behavior caused by these various termination patterns were studied by live cell imaging phase contrast microscopy and subsequent image analysis based on image thresholding and cell tracking in NIS Elements software. Using these methods we determined the number of adherent and non-adherent cells and parameters of each cell migration track on the NCD samples with O/H halves and O/H stripes.
Trvalý link: http://hdl.handle.net/11104/0238511