Stabilization of protein by freeze-drying in the presence of trehalose: a case study of tubulin

0434353 - ÚMG 2015 RIV US eng J - Článek v odborném periodiku
Dráber, Pavel - Sulimenko, Vadym - Sulimenko, Tetyana - Dráberová, Eduarda
Stabilization of protein by freeze-drying in the presence of trehalose: a case study of tubulin.
Methods in Molecular Biology. Roč. 1129, February (2014), s. 443-458. ISSN 1064-3745
Grant CEP: GA MŠMT LH12050; GA AV ČR M200521203; GA ČR GAP302/10/1701; GA ČR GPP302/11/P709
Institucionální podpora: RVO:68378050
Klíčová slova: Freeze-drying * Microtubules * Stability * Trehalose * Tubulin
Kód oboru RIV: EB - Genetika a molekulární biologie

Microtubules, polymers of the heterodimeric protein αβ-tubulin, are indispensable for many cellular activities such as maintenance of cell shape, division, migration, and ordered vesicle transport. In vitro assays to study microtubule functions and their regulation by associated proteins require the availability of assembly-competent purified tubulin. However, tubulin is a thermolabile protein that rapidly converts into non-polymerizing state. For this reason it is usually stored at −80 °C to preserve its conformation and polymerization properties. In this chapter we describe a method for freeze-drying of assembly-competent tubulin in the presence of nonreducing sugar trehalose and methods enabling evaluation of tubulin functions in rehydrated samples.
Trvalý link: http://hdl.handle.net/11104/0245524