Effect of zearalenone on reproductive parameters and expression of selected testicular genes in mice

0434230 - BTÚ 2015 RIV NL eng J - Článek v odborném periodiku
Žatecká, Eva - Děd, Lukáš - Elzeinová, Fatima - Kubátová, Alena - Dorosh, Andriy - Margaryan, Hasmik - Dostálová, Pavla - Korenková, Vlasta - Hošková, K. - Pěknicová, Jana
Effect of zearalenone on reproductive parameters and expression of selected testicular genes in mice.
Reproductive Toxicology. Roč. 45, june (2014), s. 20-30. ISSN 0890-6238. E-ISSN 1873-1708
Grant CEP: GA ČR(CZ) GAP503/12/1834; GA MŠMT(CZ) ED1.1.00/02.0109
Výzkumný záměr: CEZ:AV0Z50520701
Institucionální podpora: RVO:86652036
Klíčová slova: zearalenone * fertility * reproductive parameters in male mice * spermatogenesis * gene expression * qPCR
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 3.227, rok: 2014

We tested the effect of two different concentrations (150μg/l and 0.15μg/l) of mycotoxin zearalenone (ZEA) on the reproductive parameters and expression of testicular genes in male mice. In adult males, no reduction of body or reproductive organ weight was observed, and the seminiferous tubules were morphologically normal with ongoing spermatogenesis. However, we found decreased sperm concentration, increase of morphologically abnormal spermatozoa and increased binding of apoptotic marker annexin V. This study was also focused on the evaluation of gene expression profiles of 28 genes playing important roles during the processes occurring in the testicular tissue. We detected changes in the expression of genes important for proper spermatogenesis. Surprisingly, we observed a stronger effect after exposure to the lower dose of ZEA.
Trvalý link: http://hdl.handle.net/11104/0238381