Plant Endosomes - Methods and Protocols

0433745 - ÚEB 2015 RIV US eng M - Část monografie knihy
Malínská, Kateřina - Jelínková, Adriana - Petrášek, Jan
The use of FM dyes to analyze plant endocytosis.
Plant Endosomes - Methods and Protocols. Vol. 1209. Totowa: Humana Press, 2014 - (Otegui, M.), s. 1-11. Methods in Molecular Biology. ISBN 978-1-4939-1419-7
Grant CEP: GA ČR(CZ) GAP305/11/2476; GA ČR GPP305/11/P797
Institucionální podpora: RVO:61389030
Klíčová slova: Endocytosis * Endomembranes * FM 1-43
Kód oboru RIV: EB - Genetika a molekulární biologie
http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=MEDLINE&DestLinkType=FullRecord&UT=25117271

FM (Fei-Mao) styryl dyes are compounds of amphiphilic character that are used for the fluorescence tracking of endocytosis and related processes, i.e., the internalization of membrane vesicles from the plasma membrane (PM) and dynamics of endomembranes. Staining with FM dyes and subsequent microscopical observations could be performed both on the tissue and cellular level. Here, we describe simple procedures for the effective FM dye staining and de-staining in root epidermal cells of Arabidopsis thaliana seedlings and suspension-cultured tobacco cells. The progression of FM dye uptake, reflected by an increased amount of the dye in the endosomal compartments, is monitored under the fluorescence microscope in a time-lapse manner. The data obtained can be used for the characterization of the rate of endocytosis and the function of components of endosomal recycling machinery.
Trvalý link: http://hdl.handle.net/11104/0237919