Quantum Dots: Applications in Biology

0433692 - ÚIACH 2015 RIV US eng M - Část monografie knihy
Klepárník, Karel - Datinská, Vladimíra - Voráčová, Ivona - Lišková, Marcela
Analysis of quantum dots and their conjugates by capillary electrophoresis with detection of laser-induced luminescence.
Quantum Dots: Applications in Biology. 2nd Edition. Totowa: Humana Press Inc, 2014 - (Fontes, A.), s. 33-54. Methods in Molecular Biology, Volume 1199. ISBN 978-1-4939-1279-7
Grant CEP: GA ČR GAP206/11/2377; GA ČR(CZ) GA14-28254S
Institucionální podpora: RVO:68081715
Klíčová slova: capillary electrophoresis * quantum dots * laser-induced fluorescence * immuno assay
Kód oboru RIV: CB - Analytická chemie, separace

In many bioanalytical applications, important molecules such as DNA, proteins, and antibodies are routinely conjugated with fluorescent tags to reach an extraordinary sensitivity of analyses. Semiconductor nanoparticles, quantum dots, have already proved to be suitable components of highly luminescent tags, probes, and sensors with a broad applicability in analytical chemistry. Quantum dots provide high extinction coefficients together with a wide range of excitation wavelengths, size- and composition-tunable emissions, narrow and symmetric emission spectra, good quantum yields, relatively long size-dependent luminescence lifetime, and practically no photobleaching. Most of these properties are superior when compared with conventional organic fluorescent dyes. In this chapter, optimized procedures for the preparation of water-dispersed cadmium telluride (CdTe) quantum dots, conjugating reactions with antibodies, DNA, and macrocycles as well as their analyses by capillary electrophoresis are described. The potential of capillary electrophoresis for fast analyses of nanoparticles, their conjugates with antibodies, and immunocomplexes with targeted antigens is demonstrated on examples.
Trvalý link: http://hdl.handle.net/11104/0237857