Počet záznamů: 1  

Morphological and proteomic analysis of early stage air-liquid interface biofilm formation in Mycobacterium smegmatis

  1. 1.
    0433431 - MBÚ 2015 RIV GB eng J - Článek v odborném periodiku
    Sochorová, Zuzana - Petráčková, Denisa - Sitařová, B. - Buriánková, Karolína - Bezoušková, Silvia - Benada, Oldřich - Kofroňová, Olga - Janeček, Jiří - Halada, Petr - Weiser, Jaroslav
    Morphological and proteomic analysis of early stage air-liquid interface biofilm formation in Mycobacterium smegmatis.
    Microbiology. Roč. 160, JUL 2014 (2014), s. 1346-1356. ISSN 1350-0872. E-ISSN 1465-2080
    Grant CEP: GA AV ČR IAA500200913
    Institucionální podpora: RVO:61388971
    Klíčová slova: RHODOCOCCUS-JOSTII RHA1 * BACILLUS-SUBTILIS * GLASS-BEADS
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 2.557, rok: 2014

    We studied the early stages of pellicle formation by Mycobacterium smegmatis on the surface of a liquid medium [air liquid interface (A-L)]. Using optical and scanning electron microscopy, we showed the formation of a compact biofilm pellicle from micro-colonies over a period of 8-30 h. The cells in the pellicle changed size and cell division pattern during this period. Based on our findings, we created a model of M. smegmatis A-L early pellicle formation showing the coordinate growth of cells in the micro-colonies and in the homogeneous film between them, where the accessibility to oxygen and nutrients is different. A proteomic approach utilizing high-resolution two-dimensional gel electrophoresis, in combination with mass spectrometry-based protein identification, was used to analyse the protein expression profiles of the different morphological stages of the pellicle. The proteins identified formed four expression groups; the most interesting of these groups contained the proteins with highest expression in the biofilm development phase, when the floating micro-colonies containing long and more robust cells associate into flocs and start to form a compact pellicle. The majority of these proteins, including GroEL1, are involved in cell wall synthesis or modification, mostly through the involvement of mycolic acid biosynthesis, and their expression maxima correlated with the changes in cell size and the rigidity of the bacterial cell wall observed by scanning electron microscopy
    Trvalý link: http://hdl.handle.net/11104/0237685

     
     
Počet záznamů: 1  

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