Počet záznamů: 1
Early molecular events involved in Pinus pinaster Ait. somatic embryo development under reduced water availability: transcriptomic and proteomic analyses
- 1.0433191 - ÚEB 2015 RIV US eng J - Článek v odborném periodiku
Morel, A. - Teyssier, C. - Trontin, J.F. - Eliášová, Kateřina - Pešek, Bedřich - Beaufour, M. - Morabito, D. - Boizot, N. - Le Mette, C. - Belal-Bessai, L. - Vágner, Martin - Label, P. - Lelu-Walter, M.A. … celkem 17 autorů
Early molecular events involved in Pinus pinaster Ait. somatic embryo development under reduced water availability: transcriptomic and proteomic analyses.
Physiologia Plantarum. Roč. 152, č. 1 (2014), s. 184-201. ISSN 0031-9317. E-ISSN 1399-3054
Institucionální podpora: RVO:61389030
Klíčová slova: GERMIN-LIKE PROTEIN * ABSCISIC-ACID * HYBRID LARCH
Kód oboru RIV: EF - Botanika
Impakt faktor: 3.138, rok: 2014
Maritime pine somatic embryos (SEs) require a reduction in water availability (high gellan gum concentration in the maturation medium) to reach the cotyledonary stage. This key switch, reported specifically for pine species, is not yet well understood. To facilitate the use of somatic embryogenesis for mass propagation of conifers, we need a better understanding of embryo development. Comparison of both transcriptome (Illumina RNA sequencing) and proteome [two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis with mass spectrometry (MS) identification] of immature SEs, cultured on either high (9G) or low (4G) gellan gum concentration, was performed, together with analysis of water content, fresh and dry mass, endogenous abscisic acid (ABA; gas chromatography-MS), soluble sugars (high-pressure liquid chromatography), starch and confocal laser microscope observations. This multiscale, integrated analysis was used to unravel early molecular and physiological events involved in SE development. Under unfavorable conditions (4G), the glycolytic pathway was enhanced, possibly in relation to cell proliferation that may be antagonistic to SE development. Under favorable conditions (9G), SEs adapted to culture constraint by activating specific protective pathways, and ABA-mediated molecular and physiological responses promoting embryo development. Our results suggest that on 9G, germin-like protein and ubiquitin-protein ligase could be used as predictive markers of SE development, whereas protein phosphatase 2C could be a biomarker for culture adaptive responses. This is the first characterization of early molecular mechanisms involved in the development of pine SEs following an increase in gellan gum concentration in the maturation medium, and it is also the first report on somatic embryogenesis in conifers combining transcriptomic and proteomic datasets.
Trvalý link: http://hdl.handle.net/11104/0237423
Název souboru Staženo Velikost Komentář Verze Přístup 2014_Morel_PHYSIOLOGIA PLANTARUM_184.pdf 2 1.4 MB Jiná povolen
Počet záznamů: 1