Induction and carbon catabolite repression of phenol degradation genes in Rhodococcus erythropolis nad Rhodococcus jostii

0433025 - MBÚ 2015 RIV DE eng J - Článek v odborném periodiku
Szököl, Juraj - Rucká, Lenka - Šimčíková, M. - Halada, Petr - Nešvera, Jan - Pátek, Miroslav
Induction and carbon catabolite repression of phenol degradation genes in Rhodococcus erythropolis nad Rhodococcus jostii.
Applied Microbiology and Biotechnology. Roč. 98, č. 19 (2014), s. 8267-8279. ISSN 0175-7598. E-ISSN 1432-0614
Grant CEP: GA MŠMT 2B08062; GA ČR(CZ) GAP504/11/0394
Institucionální podpora: RVO:61388971
Klíčová slova: Phenol degradation * Phenol hydroxylase * Rhodococcus erythropolis
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 3.337, rok: 2014

Rhodococcus erythropolis CCM2595 is able to efficiently utilize phenol and other aromatic compounds. We cloned and sequenced its complete gene cluster - catA, catB, catC, catR, pheR, pheA2, pheA1 - involved in the ortho-cleavage pathway of phenol. The activity of the key enzyme of the phenol degradation pathway, two-component phenol hydroxylase, was found to be induced by phenol. When both phenol and succinate were present in the medium, phenol hydroxylase activity decreased substantially. To analyze the regulation of phenol degradation at the transcriptional level, the transcriptional fusions of the divergently oriented promoters PpheA2 and PpheR with the gfpuv reporter gene were constructed. The promoters driving expression of the genes of the pheR-pheA2pheA1 cluster were localized by determining the respective transcriptional start points
Trvalý link: http://hdl.handle.net/11104/0237348