Mekabu fucoidan: Structural complexity and defensive effects against avian influenza A viruses

0431746 - ÚOCHB 2015 RIV GB eng J - Článek v odborném periodiku
Synytsya, A. - Bleha, R. - Synytsya, Al. - Pohl, Radek - Hayashi, K. - Yoshinaga, K. - Nakano, T. - Hayashi, T.
Mekabu fucoidan: Structural complexity and defensive effects against avian influenza A viruses.
Carbohydrate Polymers. Roč. 111, Oct 13 (2014), s. 633-644. ISSN 0144-8617. E-ISSN 1879-1344
Institucionální podpora: RVO:61388963
Klíčová slova: Mekabu fucoidan * sporophyll Undaria pinnatifida * spectroscopic methods * avian influenza A virus * immunostimulating effect
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 4.074, rok: 2014

Fucoidan from the sporophyll (Mekabu) of brown seaweed Undaria pinnatifida (wakame) is interesting due to its various biological activities. Mekabu fucoidan (M-w similar to 9 kDa) of this study (MF) was previously isolated and characterized by chemical and separation methods including GPC and methylation analysis (Lee, Hayashi, Hashimoto, Nakano, & Hayashi, 2004). It was found that this fucoidan composed of partially sulphated (DS similar to 0.72) fucose and galactose at approximately equal amounts. Methylation analyses revealed complex structure of MF. However, it has been still unclear about the linkages between units and substitution patterns. To solve these structural tasks, spectroscopic methods (FTIR, FT Raman and NMR) were used in the analysis of native MF and its deesterified derivatives. According to obtained results, this polysaccharide was defined as O-acetylated sulphated fucogalactan. The defensive effects of MF were evaluated on mice infected with avian influenza A viruses (H5N3 and H7N2 subtypes); its efficacy was determined in reducing viral replication and increasing antibody production. Oral administration of MF resulted in suppressing virus yields. In addition, the production of neutralizing antibodies and mucosal IgA in the animals inoculated with the avian influenza A viruses was significantly increased. These results suggested that MF could be used for the prevention of viral infection.
Trvalý link: http://hdl.handle.net/11104/0236410