Comparison of Extraction Methods for Isolation of 20-Hydroxyecdysone from Leuzea carthamoides

0431429 - ÚCHP 2015 CZ eng C - Konferenční příspěvek (zahraniční konf.)
Machalová, Zdeňka - Sajfrtová, Marie - Sovová, Helena - Bártlová, Milena
Comparison of Extraction Methods for Isolation of 20-Hydroxyecdysone from Leuzea carthamoides.
Final program. Prague: Orgit, 2014, s. 45. ISBN 978-80-02-02555-9.
[International Congress of Chemical and Process Engineering /21./ - CHISA 2014 and Conference on Process Integration, Modelling and Optimisation for Energy Saving and Pollution Reduction /17./ - PRES 2014. Prague (CZ), 23.08.2014-27.08.2014]
Grant CEP: GA TA ČR TA01010578
Institucionální podpora: RVO:67985858
Klíčová slova: extraction methods * pressurized liquid extraction * supercritical fluids extraction
Kód oboru RIV: CI - Průmyslová chemie a chemické inženýrství

This work is focused on the extraction of 20-HE from roots and seeds of Leuzea carthamoides using SFE, PLE and Soxhlet extraction. The SFE and PLE conditions (pressure, temperature, type and concentration of solvent, extraction time) were optimized to improve extraction yields and concentrations of 20-HE in extracts. These methods were compared with a conventional Soxhlet-type extraction. The SFE experiments were carried out at temperatures from 40°C to 60°C, pressures from 30 to 65 MPa and with addition of 5-20% of co-solvent (ethanol, methanol and acetone). PLE with ethanol, methanol and acetone was made at temperatures from 70°C to 100°C, pressures from 7 to 20 MPa and extraction time from 5-15 min. Soxhlet extraction with ethanol was used to evaluate the total content of 20-HE in roots and seeds. The concentration of 20-HE in extracts was determined by HPLC.
Trvalý link: http://hdl.handle.net/11104/0236018