Evaluation of antibacterial properties of novel phthalocyanines against Escherichia coli - comparison of analytical methods

0430204 - BÚ 2015 RIV CH eng J - Článek v odborném periodiku
Mikula, Přemysl - Kalhotka, L. - Jančula, Daniel - Zezulka, Štěpán - Kořínková, R. - Černý, J. - Maršálek, Blahoslav - Toman, Petr
Evaluation of antibacterial properties of novel phthalocyanines against Escherichia coli - comparison of analytical methods.
Journal of Photochemistry and Photobiology. B - Biology Section. Roč. 138, Sep 2014 (2014), s. 230-239. ISSN 1011-1344. E-ISSN 1873-2682
Grant CEP: GA TA ČR TA01010356; GA MPO FR-TI3/196; GA ČR(CZ) GAP205/10/2280
Institucionální podpora: RVO:67985939 ; RVO:61389013
Klíčová slova: phthalocyanines * photosensitizers * antibacterial effect
Kód oboru RIV: EF - Botanika; CD - Makromolekulární chemie (UMCH-V)
Impakt faktor: 2.960, rok: 2014

We analyzed antibacterial effects of several novel phthalocyanines against E. coli and evaluated the suitability of flow cytometry for the detection of antibacterial effects of phthalocyanines in comparison with routinely used cultivation. After 3 hours of exposure under cool white light eight cationic phthalocyanines showed very high antibacterial activity in the concentration of 2.00 mg L-1 and four of them were even efficient in the concentration of 0.20 mg L-1. Antibacterial activity of neutral and anionic compounds was considerably lower or even negligible. Agglomeration of cells observed in suspensions treated with a higher concentration of certain cationic phthalocyanines affected cytometric measurements of total cell counts, therefore this parameter seems not to be fully valid in the evaluation of phthalocyanine antibacterial activity. On the other hand, cytometric measurement of cell membrane integrity appears to be a suitable and sensitive parameter.
Trvalý link: http://hdl.handle.net/11104/0236402