Arabidopsis ABCG14 protein controls the acropetal translocation of root-synthesized cytokinins

0429940 - ÚEB 2015 RIV GB eng J - Článek v odborném periodiku
Zhang, K. - Novák, Ondřej - Wei, Z. - Gou, M. - Zhang, X. - Yu, Y. - Yang, H. - Cai, Y. - Strnad, Miroslav - Liu, Ch.-J.
Arabidopsis ABCG14 protein controls the acropetal translocation of root-synthesized cytokinins.
Nature Communications. Roč. 5, FEB 2014 (2014). E-ISSN 2041-1723
Grant CEP: GA MŠMT(CZ) LO1204
Grant ostatní: GA MŠk(CZ) ED0007/01/01
Program: ED
Institucionální podpora: RVO:61389030
Klíčová slova: EQUILIBRATIVE NUCLEOSIDE TRANSPORTER * EXPRESSION ANALYSIS * MERISTEM ACTIVITY
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 11.470, rok: 2014

Cytokinins are a major group of phytohormones regulating plant growth, development and stress responses. However, in contrast to the well-defined polar transport of auxins, the molecular basis of cytokinin transport is poorly understood. Here we show that an ATP-binding cassette transporter in Arabidopsis, AtABCG14, is essential for the acropetal (root to shoot) translocation of the root-synthesized cytokinins. AtABCG14 is expressed primarily in the pericycle and stelar cells of roots. Knocking out AtABCG14 strongly impairs the translocation of trans-zeatin (tZ)-type cytokinins from roots to shoots, thereby affecting the plant's growth and development. AtABCG14 localizes to the plasma membrane of transformed cells. In planta feeding of C-14 or C-13-labelled tZ suggests that it acts as an efflux pump and its presence in the cells directly correlates with the transport of the fed cytokinin. Therefore, AtABCG14 is a transporter likely involved in the long-distance translocation of cytokinins in planta.
Trvalý link: http://hdl.handle.net/11104/0234904