SNP Discovery for mapping alien introgressions in wheat

0429596 - ÚEB 2015 RIV GB eng J - Článek v odborném periodiku
Tiwari, V.K. - Wang, S. C. - Sehgal, S. - Vrána, Jan - Friebe, B. - Kubaláková, Marie - Chhuneja, P. - Doležel, Jaroslav - Akhunov, E. D. - Kalia, B. - Sabir, J. - Gill, B. S.
SNP Discovery for mapping alien introgressions in wheat.
BMC Genomics. Roč. 15, APR 10 (2014). ISSN 1471-2164. E-ISSN 1471-2164
Grant CEP: GA ČR GBP501/12/G090; GA MŠMT(CZ) LO1204
Grant ostatní: GA MŠk(CZ) ED0007/01/01
Program: ED
Institucionální podpora: RVO:61389030
Klíčová slova: RUST-RESISTANCE GENES * TRITICUM-AESTIVUM L. * SINGLE-NUCLEOTIDE POLYMORPHISMS
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 3.986, rok: 2014

The short arm of chromosome 5M(g) of Ae. geniculata Roth (syn. Ae. ovata L.; 2n = 4x = 28, (UUMMg)-U-g-M-g-M-g) was flow-sorted from a wheat line in which it is maintained as a telocentric chromosome. DNA of the sorted arm was amplified and sequenced using an Illumina Hiseq 2000 with similar to 45x coverage. The sequence data was used for SNP discovery against wheat homoeologous group-5 assemblies. A total of 2,178 unique, 5M(g)S-specific SNPs were discovered. Randomly selected samples of 59 5M(g)S-specific SNPs were tested (44 by KASPar assay and 15 by Sanger sequencing) and 84% were validated. Of the selected SNPs, 97% mapped to a chromosome 5M(g) addition to wheat (the source of t5M(g)S), and 94% to 5M(g) introgressed from a different accession of Ae. geniculata substituting for chromosome 5D of wheat. The validated SNPs also identified chromosome segments of 5M(g)S origin in a set of T5D-5M(g) translocation lines; eight SNPs (25%) mapped to TA5601 [T5DL . 5DS-5M(g)S(0.75)] and three (8%) to TA5602 [T5DL . 5DS-5M(g)S (0.95)]. SNPs (gsnp_5ms83 and gsnp_5ms94), tagging chromosome T5DL . 5DS-5M(g)S(0.95) with the smallest introgression carrying resistance to leaf rust (Lr57) and stripe rust (Yr40), were validated in two released germplasm lines with Lr57 and Yr40 genes.
Trvalý link: http://hdl.handle.net/11104/0234681