Evolution of Tertiary Structure of Viral RNA Dependent Polymerases

0429370 - BC 2015 RIV US eng J - Článek v odborném periodiku
Černý, Jiří - Černá, B. - Valdés, James J. - Grubhoffer, Libor - Růžek, Daniel
Evolution of Tertiary Structure of Viral RNA Dependent Polymerases.
PLoS ONE. Roč. 9, č. 5 (2014), e96070. ISSN 1932-6203. E-ISSN 1932-6203
Grant CEP: GA ČR GAP502/11/2116; GA ČR GAP302/12/2490; GA MŠMT(CZ) EE2.3.30.0032
Institucionální podpora: RVO:60077344
Klíčová slova: Q-BETA replicase * C virus RNA * crystal structure
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 3.234, rok: 2014

Viral RNA dependent polymerases (vRdPs) are present in all RNA viruses; unfortunately, their sequence similarity is too low for phylogenetic studies. Nevertheless, vRdP protein structures are remarkably conserved. In this study, we used the structural similarity of vRdPs to reconstruct their evolutionary history. The major strength of this work is in unifying sequence and structural data into a single quantitative phylogenetic analysis, using powerful a Bayesian approach. The resulting phylogram of vRdPs demonstrates that RNA-dependent DNA polymerases (RdDPs) of viruses within Retroviridae family cluster in a clearly separated group of vRdPs, while RNA-dependent RNA polymerases (RdRPs) of dsRNA and +ssRNA viruses are mixed together. This evidence supports the hypothesis that RdRPs replicating +ssRNA viruses evolved multiple times from RdRPs replicating +dsRNA viruses, and vice versa. Moreover, our phylogram may be presented as a scheme for RNA virus evolution. The results are in concordance with the actual concept of RNA virus evolution. Finally, the methods used in our work provide a new direction for studying ancient virus evolution.
Trvalý link: http://hdl.handle.net/11104/0234497