Competitive incorporations of modified versus natural nucleotides by DNA polymerases

0429013 - ÚOCHB 2015 CZ eng C - Konferenční příspěvek (zahraniční konf.)
Kielkowski, Pavel - Hocek, Michal
Competitive incorporations of modified versus natural nucleotides by DNA polymerases.
Chemistry of Nucleic Acid Components. 16th Symposium. Praha: Institute of Organic Chemistry and Biochemistry AS CR, v. v. i, 2014 - (Hocek, M.), s. 295-296. Collection Symposium Series, 14. ISBN 978-80-86241-50-0.
[Symposium on Chemistry of Nucleic Acid Components /16./. Český Krumlov (CZ), 08.06.2014-13.06.2014]
Grant CEP: GA ČR GBP206/12/G151
Institucionální podpora: RVO:61388963
Klíčová slova: DNA polymerases * dNTPs
Kód oboru RIV: CC - Organická chemie

A series of 7-substituted 7-deazaadenosine and 5-substituted cytosine triphosphates were tested in competitive incorporations with different DNA polymerases with their natural analogs and analyzed using restriction endonucleases and compared with steady state kinetics of the modified and natural dNTPs. Data sets correlation is up to 99 % and gave us to find modified dATPs with high incorporation ratios.
Trvalý link: http://hdl.handle.net/11104/0234288