Electrochemistry as a Tool for an Enzyme Characterization

0428758 - ÚFCH JH 2015 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Gál, M. - Krahulec, J. - Jiríčková, K. - Sokolová, Romana - Híveš, J.
Electrochemistry as a Tool for an Enzyme Characterization.
Sborník přednášek mezinárodní odborné konference XXXIV. Moderní Elektrochemické Metody. Ústí nad Labem: Best Servis, 2014 - (Navrátil, T.; Fojta, M.; Pecková, K.), s. 40-43. ISBN 978-80-905221-2-1.
[Moderní Elektrochemické Metody /34./. Jetřichovice (CZ), 19.05.2014-23.05.2014]
Institucionální podpora: RVO:61388955
Klíčová slova: electrochemistry * Enterokinase * Impedance spectroscopy
Obor OECD: Physical chemistry

Basic biochemical properties such as Michaelis constant (KM) and turnover number (kcat) of enzyme enteropeptidase was measured by electrochemical impedance spectroscopy. Both values determined by impedance measurements are in good agreement with those obtained by traditional spectroscopic techniques. Therefore one can suppose that electrochemical methods might be successful for such measurements also in the case where usual ones (UV-Vis, fluorescence spectroscopy) are not able to determine these enzyme characteristics.
Trvalý link: http://hdl.handle.net/11104/0234055