Changes in the expression of selected testicular genes in mice

0428740 - BTÚ 2015 CZ eng K - Konferenční příspěvek (tuzemská konf.)
Valášková, Eliška - Margaryan, Hasmik - Žatecká, Eva - Pěknicová, Jana
Changes in the expression of selected testicular genes in mice.
Book of abstracts XXth Symposium of Biology and Immunology of Reproduction with international participation. Praha: Biotechnologický ústav AVČR, v.v.i, 2014 - (Pěknicová, J.), s. 1-82
[XXth Symposium of Biology and Immunology of Reproduction with international participation. Třešť (CZ), 22.05.2014-24.05.2014]
Grant CEP: GA ČR(CZ) P503/12/1834; GA MŠMT(CZ) CZ1.05/1.1.00/02.0109
Výzkumný záměr: CEZ:AV0Z50520701
Institucionální podpora: RVO:86652036
Klíčová slova: diabetes mellitus * gene expression * reproduction
Kód oboru RIV: EB - Genetika a molekulární biologie

The decrease in population fertility has become a major concern in many developed countries. Recent studies show that infertility is affecting an estimated 15% of all couples (World Health Organization, WHO, 2010). Male infertility is the primary or contributing cause in 60% of cases. Male infertility is caused by a number of factors, such as genetic background, various environmental factors and disease. Diabetes mellitus (DM), a serious health problem on its own, is also suspected to be a contributing factor to male infertility. The aim of this project was to analyze the cellular, molecular and genetic effects of diabetic environment on spermatogenesis and sperm quality and to determine the impact of DM on the in vivo reproduction, using the mouse model (Mus musculus) inbred FVB. Diabetes was induced using streptozotocin. We used our knowledge and tools (unique monoclonal antibodies developed by our group) to determine the status of reproductive organs, anogenital distance, and the quality of sperms. Genetic analysis was performed by a quantitative Reverse Transcription Polymerase Chain Reaction (qPCR). We tested selected genes which are expressed in testicular tissue and thus can influence process of spermatogenesis and consequently the sperm quality. Our preliminary data strongly suggest that DM impairs male fertility. We have found significant changes in the body and reproductive organ weight of mice with DM. We have identified qualitative and quantitative changes in the expression of proteins in epididymal fluid and sperms. We have also detected an increased number of apoptotic cells in sperm of diabetic mice compared to the control group. To our knowledge, there is no study assessing the correlation between DM and “unexplained infertility”. In view of this, it is essential to analyze the effects of DM on male fertility, sperm quality, and reproduction parameters.
Trvalý link: http://hdl.handle.net/11104/0235847