Opioid-receptor (OR) signaling cascades in rat cerebral cortex and model cell lines: the role of plasma membrane structure

0427645 - FGÚ 2015 RIV CZ eng J - Článek v odborném periodiku
Ujčíková, Hana - Brejchová, Jana - Vošahlíková, Miroslava - Kagan, Dmytro - Dlouhá, Kateřina - Sýkora, Jan - Merta, Ladislav - Drastichová, Z. - Novotný, J. - Ostašov, Pavel - Roubalová, Lenka - Parenti, M. - Hof, Martin - Svoboda, Petr
Opioid-receptor (OR) signaling cascades in rat cerebral cortex and model cell lines: the role of plasma membrane structure.
Physiological Research. Roč. 63, Suppl.1 (2014), S165-S176. ISSN 0862-8408. E-ISSN 1802-9973
Grant CEP: GA ČR(CZ) GAP207/12/0919; GA ČR(CZ) GBP304/12/G069
Institucionální podpora: RVO:67985823 ; RVO:61388955
Klíčová slova: GPCR * morphine * mu-OR, delta-OR and kappa-OR * rat brain cortex * adenylyl cyclase I and II * proteomic analysis
Kód oboru RIV: CE - Biochemie; CF - Fyzikální chemie a teoretická chemie (UFCH-W)
Impakt faktor: 1.293, rok: 2014

Proteomic analysis of plasma membrane fractions (PM) izolated from brain cortex of rats exposed to increasing doses of morphine (10-50 mg/kg) for 10 days indicated that the brain cortex of morphine-treated animals can not be regarded as being adapted to this drug as significant up-regulation of proteins functionally related to oxidative stress and alternation of brain energy metabolism occurred. The number of delta-OR was increased 2x and their sensitivity to monovalent cations altered. Our studies of plasma membrane structure and function in context with desensitization of GPCR action were extended by data indicating participation of cholesterol-enriched membrane domains in agonist-specific internalization of delta-OR. In HEK293 cells stably expressing delta-OR-Gi1alfa fusion protein, depletion of PM cholesterol was associated with decrease (by two-orders of magnitude) in affinity /potency of G-protein-response to agonist stimulation; maximum response was unchanged. Hydrophobic interior of isolated PM became more “fluid”, chaotically organized and more accessible to water molecules
Trvalý link: http://hdl.handle.net/11104/0233182