Characterization of eight Bacillus thuringiensis isolates originated from fecal samples of Fuzhou Zoo and Fuzhou Panda Center

0427463 - BC 2015 RIV KR eng J - Článek v odborném periodiku
Wu, Ch. - Wu, L. - Zhang, L. - Gelbič, Ivan - Xu, L. - Guan, X.
Characterization of eight Bacillus thuringiensis isolates originated from fecal samples of Fuzhou Zoo and Fuzhou Panda Center.
Journal of Asia - Pacific Entomology. Roč. 17, č. 3 (2014), s. 395-397. ISSN 1226-8615. E-ISSN 1876-7990
Institucionální podpora: RVO:60077344
Klíčová slova: Bacillus thuringiensis * faeces * microscophy
Kód oboru RIV: GF - Choroby, škůdci, plevely a ochrana rostlin
Impakt faktor: 0.946, rok: 2014
http://www.sciencedirect.com/science/article/pii/S122686151400034X

In eight fecal samples of 5 herbivorous animals from Fuzhou Zoo and Fuzhou Panda Center were found eight Bacillus thuringiensis isolates. Obtained isolates were characterized by crystal microscopy, cry identification, and assay against the development of Eimeria tenella oocyst in chicken embryo. Bt WCB1, WCB2, WCB3 and WCB8, originated from horse, spotted deer, giant panda and lesser panda, respectively, all exhibited bipyramidal and cuboidal crystal and harbored the same cry genes, which were cry1Cb, cry1Db, cry1Fa, cry1Ib and cry2Ab. WCB7, a Bt isolate with bipyramidal crystal from zebra, showed a different cry pattern, cry1Cb, cry1Db, cry1Fa and cry1Ia. BtWCB6, the third Bt isolate fromgiant panda, also contained bipyramidal crystal, however, harbored cry1Ib only. It was noted that Bt isolate WCB5 contained cuboidal crystal and harbored an unknown cry3 gene. It was very interesting that no cry genes were detected from Bt isolate WCB4 with dot-like crystal. Parasporal crystal proteins of WCB3, WCB4 and WCB7 obviously inhibited the development of E. tenella oocyst in chicken embryo.
Trvalý link: http://hdl.handle.net/11104/0236832