Biolistic Transfer of Hop Viroid Disease Syndrome from Slovenian Cultivar ‘Celeia’ to Czech Hop ‘Osvald’s 72’: Pathogenesis Symptoms and Identification of Dominant sequence upon Transfer of HpSVd Component

0426213 - BC 2014 RIV BE eng C - Konferenční příspěvek (zahraniční konf.)
Matoušek, Jaroslav - Radišek, S. - Jakše, J. - Duraisamy, Ganesh Selvaraj - Uhlířová, Kateřina - Orctová, Lidmila - Svoboda, P. - Patzak, J. - Rausche, J.
Biolistic Transfer of Hop Viroid Disease Syndrome from Slovenian Cultivar ‘Celeia’ to Czech Hop ‘Osvald’s 72’: Pathogenesis Symptoms and Identification of Dominant sequence upon Transfer of HpSVd Component.
Proceedings of the International Humulus Symposium /3./. Vol. 1010. Leuven: ISHS Acta Horticulturae, 2013 - (Patzak, J.; Koutoulis, A.), s. 121-128. ISBN 9789066056961.
[International Humulus Symposium /3./. Žatec (CZ), 09.09.2012-14.09.2012]
Grant CEP: GA ČR GCP501/10/J018; GA ČR GA13-03037S; GA MZe QH81052
Institucionální podpora: RVO:60077344
Klíčová slova: viroid * HSVd * Humulus lupulus L. * Real Time quantitative RT-PCR
Kód oboru RIV: GF - Choroby, škůdci, plevely a ochrana rostlin
http://www.actahort.org/books/1010/

A viroid disease syndrome that was recently described on Slovenian cultivar ‘Celeia’ was investigated. The disease was transferred using biolistic approach to Czech hop cultivar ‘Osvald’s 72’ under isolated experimental conditions. A complex of strong symptoms appeared on that was quite comparable to cultivar ‘Celeia’. Hop stunt viroid (HpSVd) was analyzed in detail in the inoculated Czech hop that survived initial infection. It was confirmed that a population of viroid was detectable in the leaves with a clearly dominant HpSVd–cucumber pale fruit viroid-like (HpSVd-cuc) sequence. Besides HpSVd, also traces of truncated Citrus viroid (CVd IV) were detected in surviving biolistically infected hop samples. It was found that experimentally induced viroid syndrome in ‘Osvald’s 72’ led to gene deregulation and especially to strong depression of HlbHLH2 transcription factor participating in metabolome regulation in hop.
Trvalý link: http://hdl.handle.net/11104/0231944