Phytoplasma: Methods and Protocols. Dodder Transmission of Phytoplasmas

0425832 - BC 2014 RIV DE eng O - Ostatní výsledky
Přibylová, Jaroslava - Špak, Josef
Phytoplasma: Methods and Protocols. Dodder Transmission of Phytoplasmas.
2013
Grant CEP: GA MZe QH91224
Výzkumný záměr: CEZ:AV0Z50510513
Institucionální podpora: RVO:60077344
Klíčová slova: Cuscuta * Catharanthus roseus * Periwinkle
Kód oboru RIV: EE - Mikrobiologie, virologie

Transmission of phytoplasmas from naturally infected plant host species using the parasitic plant Cuscuta spp. to Catharanthus roseus is an effective way to maintain a wide range of phytoplasmas for further research. Here, transmission via dodder from an infected plant Rehmannia glutinosa var. purpurea and from a symptomatic red currant plant (Ribes spp.) to C. roseus indicator plants using a “stable bridges” method is described. Typical symptoms of phytoplasma disease on periwinkle plants were obtained: virescent flowers with an increased number of axillary shoots and smaller leaves on periwinkle plants after transmission from R. purpurea, and greening flower petals (virescence) after transmission from Ribes spp. Phytoplasmas could be detected in donor and recipient plants by electron microscopy and by PCR assays using universal phytoplasma primer pairs. RFLP analyses of PCR fragments can also be used to confirm the identity of the phytoplasmas from donor and recipient plants.
Trvalý link: http://hdl.handle.net/11104/0231659