Počet záznamů: 1  

The transport of carboxylic acids and important role of the Jen1p transporter during the development of yeast colonies

  1. 1.
    0425609 - MBÚ 2014 RIV GB eng J - Článek v odborném periodiku
    Paivo, S. - Strachotová, Dita - Kučerová, Helena - Hlaváček, Otakar - Mota, A. - Casal, M. - Palková, Z. - Váchová, Libuše
    The transport of carboxylic acids and important role of the Jen1p transporter during the development of yeast colonies.
    Biochemical Journal. Roč. 454, SEP 2013 (2013), s. 551-558. ISSN 0264-6021. E-ISSN 1470-8728
    Grant CEP: GA ČR GA204/08/0718
    Institucionální podpora: RVO:61388971
    Klíčová slova: ammonia production * Jen1p protein * monocarboxylic acid import
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 4.779, rok: 2013

    On solid substrates, yeast colonies pass through distinct developmental phases characterized by the changes in pH of their surroundings from acidic to nearly alkaline and vice versa. At the beginning of the alkali phase colonies start to produce ammonia, which functions as a quorum-sensing molecule inducing the reprogramming of cell metabolism. Such reprogramming includes, among others, the activation of several plasma membrane transporters and is connected with colony differentiation. In the present study, we show that colony cells can use two transport mechanisms to import lactic acid: a 'saturable' component of the transport, which requires the presence of a functional Jen1p transporter, and a 'non-saturable' component (diffusion) that is independent of Jen1p. During colony development, the efficiency of both transport components changes similarly in central and outer colonial cells. Although the lactate uptake capacity of central cells gradually decreases during colony development, the lactate uptake capacity of outer cells peaks during the alkali phase and is also kept relatively high in the second acidic phase. This lactate uptake profile correlates with the localization of the Jen1p transporter to the plasma membrane of colony cells. Both lactic acid uptake mechanisms are diminished in sok2 colonies where JEN1 expression is decreased. The Sok2p transcription factor may therefore be involved in the regulation of non-saturable lactic acid uptake in yeast colonies.
    Trvalý link: http://hdl.handle.net/11104/0231432

     
     
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.